Minimum nucleotide sequence input is 100 kb. See PRODIGAL documentation for further details (< 100 kb will fail).
For unannotated plasmids less than 100 kb, please run PRODIGAL separately (with an appropriate training set) and upload the .faa and .gff files to PADLOC.
Genomes in .gb or .gbff format must contain full annotation details, including locus_tag and translation (protein sequence) fields.